1: Cristian Miramontes Basics of Biotechnology Biotechnology is not a new science. Humans have been growing crops and domesticating animals for many years. They have been doing these kinds of stuff since 10,000 C.E. – 4,000 C.E. They also were making many kinds of drinks and food. They baked bread, made cheese, yogurt and brewing alcohol since 2,000 C.E. This was common for many years that humans would make there own type of foods and drinks. Since DNA was discovered, Biotechnology was involved in manipulation of DNA, RNA and proteins to modify foods, animals, vaccines, and other drugs. That is why Biotechnology is not a new type of science. FDA approved first genetically modified food that tomatoes could ripen on the vine longer. Biotechnology helped in many discoveries. In the year 1797 a man named Edward Jenner injected children with smallpox vaccine. Matthias Schleiden and Theodor Schawnn stated that Plants and animals are made up of cells in 1838-1839 and in 1855 Rudolph Virchow stated that cells come from cells. Gregor Mendel said discovered that traits are passed from parents to their offspring. James Watson and Francis Crick were the first to describe the structure of DNA. Scientists injected mice to help them see how genes work. In 2003 all human genes were mapped. Biotechnology helped humans survive in different ways. One way that Biotechnology helped humans survive was with Adult Stem Cell research. Stanley Cohen and Herbert Boyer cut and pasted bacteria and human genes DNA. Viruses are biological “nanobots”. They can be as small as 20-200 nanometers. FDA patent approved to use genetically engineered insulin and first genetically engineered vaccine- hepatitis B. FDA approves first vaccine against cancer and this vaccine blocks several types of HPV.
2: Day 1: Solution and Gel Preparation
3: In this lab we learned how to calculate a specific amount of solution that is need for a lab. We also learned how to make Gels. We made a buffer to make 6 Gels. The 6 Gels that we made were made for other labs too.
5: Day 2: Micropipetting | of Electrophoresis | In this lab we injected colored dyes into the prepared gels that we made in the first lab, to see the amount of colored dye was in one test tube. We also wanted to see
6: Day 3: Restriction Digest of pARD-R
7: In this lab we injected the gels with different colored dyes to see which dye had more colors in them. Also, we wanted to see the dyes move to the positive side because the colored dyes were negatively charged.
8: Day 4: DNA Isolation of animal and plant cell
9: In this lab we wanted to extract the DNA of animal and plant cells. The DNA looks like a web like substance in the border of the juice of the plant and the ethanol.
10: Day 5: Urinalysis
11: In this lab we were able to see how to determine if a urine sample has protein and or glucose. Also we learned how to determine the pH of a urine sample.
12: Day 6: Case Study on GMOs
13: In this lab we read about genetically altered plants. We had to fill in the charts on the left based on the story. We learned some advantages and disadvantages about eating genetically altered foods.
14: Day 7: ELISA Simulation
15: In this lab we were able to find out if people have a disease with in them by using ELSIA.
16: Day 8: Forensic Mystery with Blood Cells
17: In this lab we were able to find out a persons blood type. We were able to find out that a stain that was blood or not. We also found out a suspect for the mystery and we solved the mystery too.
18: Day 9: Transformation of Ecoli with pARA-R
19: In this lab we were able to see the effect of transformation of Ecoli with pARA-R. We also were able to see to growth of Ecoli in the containers that had pARA-R.
20: Day 10: Environmental Forensics | In this lab we were able to find out if sand was full of different types of bacteria. We were also able to see if water was full of different types of bacteria.
22: Day 11: Beano Lab "Green Plastic"
23: In this lab we were able to see if the amount of glucose was able to change in different temperatures for a specific amount of time.
24: Biotechnology is Important! | *Doctors can use Biotechnology to help make our immune system stronger with vaccines. | *Biotechnology can help make fruits and vegetables last longer and won't spoil fast.
25: * Allows scientist to improve specific genes of microbes, plants, or animals . * Allows scientist to copy or edit genes. * It can help pets overcome diseases and stay healthy. * Allows plants to fight diseases and pests. * In can help what genes cause wellness or diseases. * It helps with animal breeding and growth. * Allows scientists to make genetic changes that were not possible with traditional methods. * It allows doctors help reduce allergenic reactions.
26: Reflection | 1. Please provide a detailed summary describing the artifact and its purpose. This artifact is a scrapbook I made during ESY. The purpose of this project is to show pictures and information about the kind of labs we did over ESY. In our scrapbook we had to include pictures and our work on the labs on each page. On the pages we had to include the title of the lab, the purpose, pictures, and our work we did during the labs. We also had to write an essay on how Biotechnology affects our lives. 2. Identify and list the GLPO(s) that were satisfied in completing this artifact. In addition, please explain how these GLPO(s) were met by specifically referring to examples or evidence from the artifact. (Please specifically refer to the indicators within each domain, and do not simply state that you “Took Action or Communicated Ideas.”) In this project I communicated ideas with my classmates and teacher. Another GLPO(s) that was used was Taking Actions. I took action by making this scrapbook with information I gathered from the labs we did. Also I used Recognizing Perspectives because some people support the idea of eating genetically altered food and others are against it. Scientist made many discoveries do to Biotechnology. It also made diseases easier to find a cure to them. Other countries don’t have the knowledge that can help them improve their environment.
27: 3. What skills and concepts were learned in completing this artifact and how will they affect your future? By doing this project I learned many new skills. I learned how to isolate DNA in animal and plant cells. I also learned how to prepare gels to separate colored dyes using an electrophoresis. Another skill that I learned was how to determine a blood type for a crime scene. Also I learned how to if out if a person has a disease within them. This will help me later on in the future if I decide to work in a crime forensics. Also it will help me when I’m working in crime forensics I can determine a blood type easier and faster than just not knowing what to do. 4. What might you do differently if you were able to recreate or revise the final product? If I was able to recreate this project I will fix some of the pictures that are in the scrapbook. I would fix it because some of the pictures with show some of the work we were doing. I would also like to make some of the pictures of the work more clear. I would make it clearer because I want the people that are looking through my scrapbook see the work we did during the labs. Another thing I would like to fix is the color font. I would like to fix the color font because some of the colors didn’t make the words stand out in the background.